Products

Bioventix now provides ELISA Kits to monitor human exposure to industrial pollutants.

Pyrene (HOP-G) ELISA Kit

Pyrene is a polycyclic aromatic hydrocarbon (PAH) commonly formed during the incomplete combustion of organic matter. When pyrene enters the body it is metabolised to form 1-hydroxypyrene (1-HOP) and subsequently 1-hydroxypyrene glucuronide (HOP-G) for urinary secretion. HOP-G is therefore an important biomarker for human exposure to airborne PAH pollutants.

This kit is a sandwich enzyme-linked immunosorbent assay kit for the detection of 1-hydroxypyrene glucuronide (HOP-G) in urine samples. Sufficient for 40 samples run in duplicate.

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Data Summary

Excel Result Template

Information for Use

Product details

No. of Plates

1 x 96-well

Samples/Plate

40 samples in duplicate

Sample Types

Urine

Kit Contents

  • Pre-coated and blocked immunosorbent 96-well plate (x1)
  • HOP-G positive control (1.5 mL at 2000 pg/mL)
  • Diluent Buffer (13 mL)
  • Conjugate solution (13 mL)
  • Substrate solution (13 mL)
  • Stop solution (13 mL)
  • 10 x Wash buffer concentrate (55 mL)

Assay Range

22 – 220 pg/mL

Sensitivity

22 pg/mL

Absorption Wavelength

450 nm

Storage Conditions

2 – 8 °C

Standard Curve